w88 slot
Illumina sequencing
We offer Illumina library preparation w88 slot using commercially available kits. We have two Illumina sequencing instruments: NextSeq 2000 and MiniSeq. Our sequencing output has a range of 8 million to 1.8 billion read/read pairs, or 2.4 to 540 gigabases. This is the equivalent of 0.6 to 110 human genome equivalents.
| Instrument | Kit type | Cycles | Advertised Gb | Cost/Gb | Cost/1M reads/read pairs | Human Genome equivalents |
|---|---|---|---|---|---|---|
| NextSeq 2000 | P1 | 100 | 10 | .30 | .84 | 3.3 |
| NextSeq 2000 | P1 | 300 | 30 | .67 | .50 | 10 |
| NextSeq 2000 | P1 | 600 | 60 | .07 | .44 | 20 |
| NextSeq 2000 | P2 | 100 | 40 | .94 | .59 | 13.3 |
| NextSeq 2000 | P2 | 200 | 80 | .38 | .88 | 26.7 |
| NextSeq 2000 | P2 | 300 | 120 | .55 | .46 | 40 |
| NextSeq 2000 | P2 | 600 | 240 | .73 | .04 | 80 |
| NextSeq 2000 | P3 | 100 | 120 | .46 | .85 | 40 |
| NextSeq 2000 | P3 | 200 | 240 | .78 | .56 | 80 |
| NextSeq 2000 | P3 | 300 | 360 | .36 | .41 | 120 |
| NextSeq 2000 | P4 | 50 | 90 | .00 | >655_word_end<.95 | 30 |
| NextSeq 2000 | P4 | 100 | 180 | .25 | .43 | 60 |
| NextSeq 2000 | P4 | 200 | 360 | .08 | .02 | 120 |
| NextSeq 2000 | P4 | 300 | 540 | .55 | .57 | 180 |
| Instrument | Kit type | Cycles | Sequencing kit cost (w/ shipping) | Advertised reads/read pairs (millions) | Advertised Gb | Cost/Gb | Cost/1M reads/read pairs | Human Genome equivalents |
|---|---|---|---|---|---|---|---|---|
| MiniSeq | Rapid high output | 100 | ,083.53 | 20 | 2 | 8.75 | .88 | 0.7 |
| MiniSeq | High output | 75 | 9.60 | 25 | 1.88 | 4.89 | .96 | 0.6 |
| MiniSeq | High output | 150 | ,109.71 | 25 | 3.75 | 2.33 | .85 | 1.3 |
| MiniSeq | High output | 300 | ,780.38 | 25 | 7.5 | 8.50 | .55 | 2.5 |
| MiniSeq | Mid output | 300 | 4.41 | 8 | 2.4 | 3.44 | .03 | 0.8 |
* Yields are as advertised by Illumina. Results will vary.
Contact info and w88 casino games to begin an Illumina sequencing project.
Sanger sequencing
The Nevada Genomics Center operates a SeqStudio Genetic Analyzer for Sanger sequencing. All of our runs include an internal plasmid control to aid in quality control.
Sanger results (.AB1 files) will be returned to clients in 24-48 hours.
- Follow the w88 online casino sequencing: Optimal amount of template and primer.
- We request a minimum volume of 5uL; if your w88 slot is less than 5uL please add water to bring the submitted w88 slot up to 5uL. The maximum volume we accept is 25uL, so if your template DNA is too dilute please concentrate it.
- Submitted w88 slot should be in water. EDTA is common in elution buffers and will inhibit the Sanger reaction.
- Do NOT use DEPC-treated water for any dilutions involved in Sanger w88 slot preparation.
- For PCR amplicons:
- Ensure only one PCR product is present (visualize with a gel).
- PCR reactions must be cleaned up prior to sequencing using routine cleanup kits. We offer PCR cleanup w88 slot.
- Use a dsDNA-specific quantification method (e.g. Qubit, picogreen) to quantify your templates. We offer quantification w88 slot.
- How to submit your w88 slot:
- Using 8-well strip tubes:
- Label each tube with the w88 slot number (1, 2, 3, …).
- Write the order number on the side of the first tube of the strip.
- Using 96-well plates:
- Submit a maximum of 96 w88 slot per order if you are using plates.
- Order w88 slot down columns. Sample 1 is A1, sample 2 is B1, etc.
- Write the order number on the plate.
- Email the Sanger submission form to the Nevada Genomics Center prior to dropping off w88 slot.
- w88 slot may be physically submitted to ARF 408, or mailed to the Nevada Genomics Center.
- There is a Sanger drop-off basket in the hallway if the laboratory is closed. w88 slot will be stable if left overnight.
- Using 8-well strip tubes:
Fragment analysis/genotyping/DNA fingerprinting
Fragment analysis can be performed using microsatellite or RFLP techniques. w88 slot are run on the SeqStudio Genetic Analyzer, which is equipped with filters compatible with 6-FAM, VIC, NED, PET and LIZ (for size standards).
We can run plates provided as-is, or prepare your w88 slot by diluting (if necessary) and adding size standard (LIZ 600).
We will determine the best dilution for your w88 slot if necessary for a nominal fee.
- Ensure that your w88 slot prep process (PCR) is working optimally and robustly. We recommend being able to dilute PCRs by at least 100-fold for optimal capillary runs.
- How to submit your w88 slot:
- Using 8-well strip tubes:
- Label each tube with the w88 slot number (1, 2, 3,…).
- Write the order number on the side of the first tube of the strip.
- Using 96-well plates:
- Submit a maximum of 96 w88 slot per order if you are using plates.
- Order w88 slot down columns. Sample 1 is A1, sample 2 is B1, etc.
- Write the order number on the plate.
- Using 8-well strip tubes:
PCR cleanup
We offer PCR cleanup for Sanger sequencing w88 slot. Your samples will be quantified and an appropriate amount is used in an enzymatic reaction that degrades remaining PCR primers and dephosphorylates remaining dNTPs. This treated sample can then be used directly in Sanger sequencing.
How to submit your w88 slot:
- Using 8-well strip tubes:
- Label each tube with the w88 slot number (1, 2, 3,…).
- Write the order number on the side of the first tube of the strip.
- Using 96-well plates:
- Submit a maximum of 96 w88 slot per order if you are using plates.
- Order w88 slot down columns. Sample 1 is A1, sample 2 is B1, etc.
- Write the order number on the plate.
Quantification w88 slot
We use quantification methods specific for dsDNA or RNA.
Up to 48 w88 slot will be quantified with a Qubit fluorometer.
A plate-based assay will be used to quantify 48 or more w88 slot. Standards are measured in triplicate and you can request multiple measurements of your w88 slot.
We must be able to pipet a minimum of 2 ul of your w88 slot; provide 3 ul.
How to submit your w88 slot:
- Using 8-well strip tubes:
- Label each tube with the w88 slot number (1, 2, 3,…).
- Write the order number on the side of the first tube of the strip.
- Using 96-well plates:
- Submit a maximum of 96 w88 slot per order if you are using plates.
- Order w88 slot down columns. Sample 1 is A1, sample 2 is B1, etc.
- Write the order number on the plate.
You will receive a spreadsheet listing the concentration of your w88 slot. If you requested a plate-based assay for quantification, the data, including standard curve and subsequent calculations, will be included.
Agilent TapeStation 4150 w88 slot
The Agilent TapeStation 4150 can be used to determine the size range of DNA or RNA samples. RIN numbers are reported for RNA samples. We are currently offering three types of ScreenTapes as part of our regular w88 slot. Please contact us if you are interested in the High Sensitivity versions of the DNA & RNA ScreenTapes, as well as cell-free and genomic (up to 60 kb) DNA ScreenTapes.
TapeStation D1000
w88 slot should be 0.1 to 50 ng/ul. The dynamic size range is 35 to 1,000 bp.
TapeStation RNA
w88 slot should be 25 to 500 ng/ul.
Runs require 1-2 uL of sample, but please submit a minimum 3uL aliquot so that we may accurately remove a 1-2 uL aliquot. We can process any number of w88 slot. Please submit w88 slot in strip tubes.
A PDF report with the results will be emailed to you.
QuantStudio 3 w88 slot
We have a QuantStudio 3 that can be used with 0.2 ml PCR plates.
Contact info and w88 casino games to discuss use of the QuantStudio 3. In general, you will need to prepare plates yourself and bring them to the genome center to run. You will be able to easily set up and access your qPCR runs via ThermoFisher Connect, a cloud-based application.