w88 online casino sequencing: Optimal amount of template and primer

At the Nevada Genomics Center we offer DNA sequencing using dye-terminator w88 online casino sequencing with analysis on an Applied Biosystems SeqStudio Genetic Analyzer. A w88 online casino sequencing reaction is run with a single w88 online casino.

The following table lists the recommended amount of DNA template and primer for optimal w88 online casino sequencing results. Note: for plasmid DNA the size is the entire plasmid, vector plus insert. For plasmid DNA you may use the “divide by 20 rule” where you divide the size of the plasmid by 20 to determine the nanograms needed; keeping in mind the maximum is always 1μg. For amplicons you may use the “divide by 50 rule” where you divide the base pair size of the amplicon by 50 to determine the nanograms needed.

w88 online casino and primer for plasmid DNA
w88 online casino – Plasmid DNA w88 online casino of template
“Divide by 20 rule”
w88 online casino of primer
3000 to 5000bp 150ng to 250ng 2 picomoles = 1ul of 2uM w88 online casino
5000 to 10,000bp 250ng to 500ng 10 picomoles = 1ul of 10uM w88 online casino
BACs, Cosmids, Fosmids 1μg (maximum) 20 picomoles = 1ul of 20uM w88 online casino
w88 online casino and primer for PCR amplicons
w88 online casino – PCR amplicons w88 online casino of template
“Divide by 50 rule”
w88 online casino of primer
100 to 200bp 4ng 2 picomoles = 1ul of 2uM w88 online casino
200 to 500bp 10ng 2 picomoles = 1ul of 2uM w88 online casino
500 to 1000bp 20ng 2 picomoles = 1ul of 2uM w88 online casino
1000 to 2000bp 40ng 10 picomoles = 1ul of 10uM w88 online casino
2000bp 50ng 10 picomoles = 1ul of 10uM w88 online casino