w88 online casino sequencing: Optimal amount of template and primer
At the Nevada Genomics Center we offer DNA sequencing using dye-terminator w88 online casino sequencing with analysis on an Applied Biosystems SeqStudio Genetic Analyzer. A w88 online casino sequencing reaction is run with a single w88 online casino.
The following table lists the recommended amount of DNA template and primer for optimal w88 online casino sequencing results. Note: for plasmid DNA the size is the entire plasmid, vector plus insert. For plasmid DNA you may use the “divide by 20 rule” where you divide the size of the plasmid by 20 to determine the nanograms needed; keeping in mind the maximum is always 1μg. For amplicons you may use the “divide by 50 rule” where you divide the base pair size of the amplicon by 50 to determine the nanograms needed.
w88 online casino – Plasmid DNA | w88 online casino of template “Divide by 20 rule” |
w88 online casino of primer |
---|---|---|
3000 to 5000bp | 150ng to 250ng | 2 picomoles = 1ul of 2uM w88 online casino |
5000 to 10,000bp | 250ng to 500ng | 10 picomoles = 1ul of 10uM w88 online casino |
BACs, Cosmids, Fosmids | 1μg (maximum) | 20 picomoles = 1ul of 20uM w88 online casino |
w88 online casino – PCR amplicons | w88 online casino of template “Divide by 50 rule” |
w88 online casino of primer |
---|---|---|
100 to 200bp | 4ng | 2 picomoles = 1ul of 2uM w88 online casino |
200 to 500bp | 10ng | 2 picomoles = 1ul of 2uM w88 online casino |
500 to 1000bp | 20ng | 2 picomoles = 1ul of 2uM w88 online casino |
1000 to 2000bp | 40ng | 10 picomoles = 1ul of 10uM w88 online casino |
2000bp | 50ng | 10 picomoles = 1ul of 10uM w88 online casino |