Cellular and Molecular Imaging w88 online sports betting

Location: FA 312 (door code access required)

Two Biorad real-time PCR machines combine advanced optical technology with precise thermal control to deliver sensitive, reliable detection, quick set-up runs and monitoring of amplification traces in real-time on the integrated LCD touch screen. Directly adjacent to the two qPCR detection systems, there is a PC computer containing easy to use w88 online sports betting for on-site analysis of qPCR data.

Key features include:

• w88 online sports betting to 5-target multiplexing with sample volumes as low as 10 μl
• Thermal gradient feature
• Visualize all run w88 online sports betting at once and export only the w88 online sports betting you need in the format you want

Location: FA 312 (door code access required)

The Biorad Chemidoc is an easy, high-performance gel and blot w88 online sports betting for accurate quantification of protein samples in gels and membranes. The Chemidoc streamlines Western blotting in a five-step approach by combining traditional blotting techniques with innovative tools to allow improved band quantitation and quick assessment of electrophoresis results and blot transfer quality prior to western blotting. The Chemidoc enables rapid western blot results in as little as 15 min with efficient protein transfer in 3-minute and 5-minute stain-free gel w88 online sports betting. The embedded software includes all the tools for quantitative and qualitative image and data analysis. Images taken on the ChemiDoc can also directly be printed.

Key features include:

• Electrophoresis applications: protein including stain free and DNA gel w88 online sports betting
• Blotting applications: identify and quantify proteins, DNA, protein-ligand interactions, etc. with chemiluminescence or colorimetric detection
• Internal computer with Image Lab Touch w88 online sports betting
• Automatic recognition of application-specific tray and adjustment of w88 online sports betting parameters and software options
• Illumination sources: Trans-UV, 302 w88 online sports betting excitation, Epi-white, Trans-white (requires White sample tray provided), Trans-blue 450-490 w88 online sports betting excitation (requires Blue sample tray provided)
• Detection: chemiluminescence filter 590/110 nm standard emission filter for protein and DNA gel and blot w88 online sports betting.
• Detector: cooled CCD camera, 6 megapixels
• Exports w88 online sports betting to a USB drive as SCN, TIFF, JPEG files

Location: FA 314 (door code access required)

The CellDrop FL is an automated w88 online sports betting counter that features a DirectPipette technology to eliminate plastic slides and cumbersome hemocytometers from routine w88 online sports betting counting. The CellDrop FL comes with dual channel fluorescence and brightfield optics, variable height sample chamber, and easy-to-use analysis software. It enables fast w88 online sports betting counts, viability assessments, and GFP transfection efficienty measurements across the widest range of w88 online sports betting density, w88 online sports betting type and application.

Location: FA 314 (door code access required)

The EVOS M5000 w88 online sports betting system from ThermoFisher Scientific is a fully integrated digital inverted microscope for fluorescence, color and transmitted light for tissue culture dishes and post-cell sorting applications. It includes 3 filter cubes for w88 online sports betting DAPI, Texas Red and GFP-labeled cells, and a high-resolution CMOS camera to capture quality color images of cells post-sort. In essence, the microscope would allow high-quality images and a rapid assessment of fluorescently labeled cells post-sort.

Key features include:

• Objectives: 1.25x (PLAN APO), 10X (LWDPH 0.30NA/ 7.13WD), 40X (LWDPH 0.65NA/ 1.79WD)
• Filter sets: GFP, TXR, DAPI

Location: FA 312 (door code access required)

The Typhoon Phosphoimager is a fast and versatile laser scanner for sensitive and quantitative measurements of radio-isotropic-labeled samples. The Typhoon is a non-confocal variable mode laser scanner offering high-speed and resolution for precise quantitation of proteins, nucleic acids and other biomolecules. The scanner comprises "filmless" autoradiography with storage phosphor screens. The laser can generate 16-bit images at up to 25 μm pixel resolution to give precise quantitation in gels and blots, with a high speed to scan gels of up to 240 x 250 mm in less than 2 min. Two phosphor screens with a size of 200 X 250 mm are available for autoradiography. The scanner can detect a broad range of isotopes in radiolabeled samples. Directly adjacent to the phosphoimager system, there is a PC computer with a w88 online sports betting package that allows on-site quantitative and qualitative analysis of images and data.

Key features include:

• Applications: radioisotopic labels by storage phosphor, chemifluorescent Western blots, and single fluorescence, as well as digitization of colorimetric stains (e.g., Coomassie™ Blue and silver-stained gels)
• Resolution: 16-bit images are generated at w88 online sports betting to 25 µm pixel resolution
• Speed: 24 x 25 cm gel w88 online sports betting scanned in less than two minutes at 100 µm resolution
• Four lasers: blue (473 w88 online sports betting), green (532 w88 online sports betting), red (635 w88 online sports betting), and red (650 w88 online sports betting)
• Radioisotopes: ³H,¹⁴C,³²P,³³P,³⁵S
• Max scanning area: phosphorimaging: 20 × 40 cm, fluorescence: 24 × 40 cm

Location: FA 313

The Integra media autoclave allows the rapid and gentle preparation of a 1-10 liter culture medium. Precise control of temperature, time and pressure during the sterilization process guarantees constant high quality. The mediaclave w88 online sports betting used for the preparation and sterilization of numerous types of culture media, including media forC. elegansresearch. The wide adding port facilitates the safe addition of supplements prior to dispensing. The mediaclave is connected to a peristaltic pump housed in a vertical flow workstation to fill Petri dishes and vials.

Key features include:

• Rapid and gentle preparation of 1-10 liter culture medium
• Large and strong magnetic stirrer with adjustable speed and reversing rotation direction ensures homogenous media preparation over a wide viscosity range
• Pt-1000 temperature probe and microprocessor control of process parameters.
• Fast heating and rapid cooling
• Peristaltic pump DOSE-IT housed in an AirClean vertical flow workstation w88 online sports betting used to fill containers of unusual volumes or shapes, i.e. Petri dishes, bottles, flasks

Location: FA 324 (door code access required)

The Leica TCS SP8 LIGHTNING confocal allows image information extraction with super-resolution acquisition that exploits the sub-diffraction lateral resolution of confocal microscopy. It allows to capture cellular details and observe dynamics with a resolution down to 120 w88 online sports betting, which is close to STED super-resolution microscope capabilities. The upright confocal is capable of simultaneous collection from up to 3 detection channels (two PMTs, one HyD) using multiple laser lines (diode 405, blue 488, green 552, red 638 w88 online sports betting). The confocal is configured with a conventional tandem scanner (8 kHz), a super Z-galvo stage type S, multiple objectives (10x, 20x, 25x, 40x, 63x), and stands on an anti-vibration table. The confocal is connected to a computer workstation equipped with Leica LAS-X software and a 30-inch monitor.

Key features include:

• DM 6000 upright with EL 6000 fluorescence
• Super Z Galvo stage, Type S
• Objectives: 10x (HC PL FLUOTAR 10x/0.30), 20x (HC PL APO 20x/0.7 IMM CORR CS2), 25x (HCX IRAPO L 25x/0.95 W 0.17), 40x (HC PL APO 40x/1.30 Oil CS2), and 63x (HC PL APO 63x/1.40 Oil CS2)
• Tandem resonant scanner 8 kHz
• 2 PMT SP confocal detectors for w88 online sports betting
• 1 HyD SP GaAsP detector for gated w88 online sports betting
• Lasers: 405 w88 online sports betting DMOD Compact, Port UV/405, Blue 488 w88 online sports betting, Green 552 w88 online sports betting, Red 638 w88 online sports betting
• LAS-X navigator w88 online sports betting
• LIGHTNING work station

Location: FA 324

THUNDER imagers are a brand-new class of computational clearing high-resolution wide-field microscopes developed exclusively by Leica Microsystems. These imagers can acquire sharp data and high-quality images by instant removal of out-of-focus blur or haze inherent to thick 3D samples that are generally acquired using widefield microscopy. These imagers also significantly shorten the w88 online sports betting workflow from image acquisition to data analysis with the new LAS-X navigator software. The THUNDER Imager 3D Tissue is a motorized upright microscope and is outfitted with an ultra-fast DFC 9000 GT/C camera, and is ideal for cell culture, tissues, fixed samples, and cleared tissues. The THUNDER Imager Model Organism is an M205 FCA stereomicroscope intended to image embryos, whole organs and small organisms.

Key features of the THUNDER 3D Tissue imager include:

• Objectives: 5x (HC PL FLUOTAR 5x/0.12), 10x (HC PL FLUOTAR 10x/0,32), 20x (HC PL FLUOTAR 20x/0.55), 63x (HC PL FLUOTAR 63x/1.30 oil)
• Scanning stage, DM4-6, 100x100, 2 w88 online sports betting
• Stage insert 2 slides 3”x2’, 76x52 w88 online sports betting
• Filter sets: GFP, RHOD, CY 5, and DAPI
• DFC 9000 GT/C Andor Zyla sCMOS camera
• 1 TB SSD RAID for LAS X workstation with 32” monitor

Key features of the THUNDER Model Organism imager include:

• Objectives: 1x (PLAN APO)
• XY-scanning stage 150x100 w88 online sports betting
• Handwheel-Motor Focus for M-series and foot switch
• Filter sets: GFP, TXR, CY 5 and DAPI
• DFC 9000 GT/C Andor Zyla sCMOS camera
• 1 TB SSD RAID for LAS X workstation with 32” monitor

Location: FA 312 (door code access required)

The Nanophotometer N50 allows the rapid assessment of DNA, RNA and protein concentrations of small (1 µl) w88 online sports betting. It is a stand-alone instrument for nucleic acid quantification and purity check (dsDNA, ssDNA, RNA, mRNA, miRNA, Oligos including dye-labeled w88 online sports betting)) and for protein quantitation and purity check (BSA, Antibodies, IgG, IgE, Serum Albumin, etc., including dye-labeled w88 online sports betting), small molecules in organic solvents, kinetics in a drop, and more. It has an interactive touch screen, and a built-in high performance computer processor and sufficient memory to provide a powerful and easy storage of methods and data.

Location: FA 314 (door code access required)

The Sartorius Octet R8 label-free protein analysis system provides fast, high throughput, and accurate characterization for biomolecules such as proteins, antibodies, peptides, DNA, RNA, liposomes, viruses and various media. It performs quantitation and kinetic analysis of up to 96 samples in 30 min to 2.5 hours, depending on the specific assay. It w88 online sports betting used for a wide range of analyses, including kinetic analysis, titers IgG and other proteins, reagent quantification, crude antibody screening, epitope binning/mapping, ligand binding assays, infectious disease monitoring, and more. Analysis w88 online sports betting done using a single channel or up to 8 channels, enabling flexibility in sample throughput.

Note: Please acknowledge the funding source NIH S10 OD032385 in publications and presentations when using this instrument. Users must purchase their own consumables

Key features include:

• Equipped with w88 online sports betting plate cooling for temperature-sensitive protein and assays
• Single channel or w88 online sports betting to 8 channels
• Integrative acquisition and analysis w88 online sports betting

Location: FA 312 (door code access required)

The QIAgility is a compact benchtop instrument that enables rapid, high-precision setup of PCR experiments with ready-to-use w88 online sports betting for ease and convenience. The w88 online sports betting provides step-by-step guidance for worktable setup and automatic calculation of all mixes, eliminating the need to program pipetting steps. Just select the kit and cycler, define your targets and start the run. For added flexibility, the QIAgility is operated via a laptop computer.

Key features include:

• Highly suited for laboratories performing PCR and RT-PCR reactions with applications in gene expression, cloning, genotyping and more
• The instrument uses 50 µl and 200 µl Conductive Filtered Tips for liquid-level sensing
• Accessories included: 2 x Adapter, 96 x 0.2 ml PCR Tubes 1 x Adapter, Flip-Cap 32 x 1.5 ml Tapered Base 1 x Adapter, 72 x 0.1 ml Strip Tubes 1 x Master Mix Block, 5 x 5 ml 1 x Master Mix Block, 1 x 5 ml, 4 x 2 ml 1 x Master Mix Block, 1 x 5 ml, 4 x 1.5 ml 1 x Reagent Block, 16 x 0.2 ml / 2 ml Flat / 1.5 ml Tapered Tubes 4 x Tip Rack Holder for 96-Tip Rack.
• HEPA filter: preinstalled dual-cartridge 3M Air-Mate HEPA filter w88 online sports betting provides positive pressure clean air within the closed hood
• Preinstalled UV lamp for workable decontamination
• Operating temperature, 15–30 °C (59–86°F)

Location: FA 314 (door code access required)

The Qubit 4 Fluorometer accurately measures DNA, RNA, and protein quantity. The Qubit together with an RNA IQ Assay Kit w88 online sports betting accurately distinguish intact from degraded RNA in just two easy steps. For all Qubit assays, the concentration or quality of the target molecule in the sample is reported by a fluorescent dye that emits a signal only when bound to the target, which minimizes the effects of contaminants on the result. The easy-to-use touch-screen menus make it easy to select and run the assays you need, with results displayed in just a few seconds.

Key features include:

• Quantitation of DNA, RNA, and protein in less than three seconds per w88 online sports betting
• Measures intact RNA in less than 5 seconds per w88 online sports betting
• High levels of accuracy using only 1–20 μL of sample, even with very dilute w88 online sports betting.
• Stores result from w88 online sports betting to 1000 samples
• Large 5.7-inch, state-of-the-art color touch screen for easy workflow navigation
• Graphical display indicates when w88 online sports betting are in the extended range or out of range
• Exports w88 online sports betting to a USB drive or directly to your computer via a USB cable

Location: FA 318 (door ‘key’ access and laser certification required)

Two-photon microscopy has become an indispensable tool for cellular neuroscience, allowing high-resolution fluorescent w88 online sports betting of cells in vitro and in vivo living brains and whole animals. The HyperScope system is a custom-build, two-photon microscope equipped with a Mai-Tai eHPDS broadly tunable Ti-sapphire laser (690-1040 nm), multiple objectives (16x, 20x and 40x), and stands on a Thorlabs breadboard and anti-vibration table. With the Scientifica SciCam Pro CCD camera, it is possible to perform functional w88 online sports betting of video frame rates of 24 fps (full resolution) and 40 fps (binned 2 x 2). The detector assembly includes one red-shifted PMT and one GaAsP PMT module, and epifluorescence w88 online sports betting with filter sets for GFP and mCherry. The scan mirrors in the w88 online sports betting path can be arranged in multiple configurations depending on your needs. With up to 3 mirrors in series, you can choose between galvo/galvo (GG), resonant/galvo (RG), or resonant/galvo/galvo (RGG) arrangements. The microscope is fitted with an ultra-smooth Z-axis drive to ensure vibration-free movement, with the Z-axis being controlled with software for acquiring z-stacks with a minimum step size of 0.1 mm. The addition of a Piezo z-axis objective positioner allows volume w88 online sports betting up to 400 mm. The microscope can be easily configured and switched between in vitro and in vivo applications by removing the substage optics and riser block. The system is enclosed in a black-out cage to prevent light and noise pollution of biological w88 online sports betting.

This platform includes SciScan acquisition w88 online sports betting, allowing users to integrate SciScan easily with custom-written applications, such as LabView and ScanImage. The ScanImage w88 online sports betting interface used by Scientifica was developed by HHMI Janelia Farm specifically for neuroscience applications and is considered to be the most advanced w88 online sports betting for two-photon laser scanning on the market.

Key features include:

• SliceScope upright w88 online sports betting with motorized frame sitting on top of a Thorlabs breadboard with tapped holes on top of a TMC Cleantop Optical Table
• Motorized positioning of objective and condenser in Z-axis, controlled by PatchPad
• Motorized Movable Base Plate for use with riser block and in vitro applications
• HyperScope Scan Path including w88 online sports betting to three scanning mirrors
• HyperScope X-Galvo module
• HyerpScope Resonant X module (8 kHz resonant galvanometer)
• Pockel w88 online sports betting (680-1100 nm) with 500:1 extinction ratio
• Workstation and data acquisition, which enables resonant w88 online sports betting (with SciScan) and RG or RGG system for resonant and/or galvo w88 online sports betting using ScanImage
• Detectors: 1 red-shifted PMT module, and 1 multi-photon GaAsP PMT module
• Objectives include: 16x (CFI75 LWD 16xW N.A. 0.80, W.D. 3.0 w88 online sports betting), 20x (XLUMPLFLM 20xW, N.A. 1.0, WD 2.0 w88 online sports betting, water immersion), 40x (LUMPPLFLN 40xW, N.A. 0.8, W.D. 3.3mm, water immersion)
• The w88 online sports betting is compatible with RMS, M25, M27 and M32 threaded objectives allowing users to easily interchange objectives
• Filter sets: GFP (470/40x, 495LP, 525/50m), mCherry (560/40x, 585LP, 630/75m)
• SciCam Pro CCD camera with 24 fps at full resolution (40 fps binned 2 x 2)
• 15x eye-piece with FOV of 14.5mm and tiltable nose piece
• Piezo Objective Z-drive allows fast acquisition of volumes w88 online sports betting to 400 microns travel for large and small objectives
• Laser: Mai-Tai eHP DS-244 Ti-sapphire oscillator (690 to 1040 w88 online sports betting, 2.4W average power, ~80 MHz repetition rate, <70 fs pulse width)

Location: FA 312 (door code access required)

The GeneSys spectrophotometer performs high-throughput quantitative UV-Vis measurements where a double beam is required as a reference w88 online sports betting position. Optimized for usability and performance, this system features a high-resolution color touchscreen and optional Wi-Fi networking. The compartment is an 8-position w88 online sports betting changer (standard).

Key features include:

• Double Beam/Reference w88 online sports betting
• Standard 8-position w88 online sports betting changer with switchable turret
• Room light resistance for 'open-lid' operation
• Accommodates cells w88 online sports betting to 100 mm pathlength
• Exports w88 online sports betting to a USB drive or directly to your computer via a USB cable

Location: FA 314 (door code access required)

The Trans-Blot Turbo transfer w88 online sports betting combines traditional blotting techniques with modern filter paper and buffers allowing rapid transfer of proteins from gels to membranes with minimal preparation time. The w88 online sports betting integrates a high amperage power supply that directs current between a built-in platinized titanium anode and a stainless steel cathode.

Key features include:

• Two cassettes with rapid transfer of 1-4 mini or 1-2 midi gels in 3 min in a single run

Location: FA 314 (door code access required)

The NanoCellect WOLF G2 w88 online sports betting sorter is used for a variety of applications, including antibody discovery, single w88 online sports betting sorting and plating, genomics, single-w88 online sports betting RNA sequencing, plant biology, gene editing, w88 online sports betting line development, and discovering microorganisms in ocean or pond water. The WOLF G2 is designed to gently sort viable, healthy cells using a sterile cartridge-based microfluidics system. The WOLF G2 w88 online sports betting sorter is ideal for tasks like w88 online sports betting line development and sorting plant protoplasts, as well as for monoclonal outgrowth of iPSCs and microalgae. At < 2 psi, the WOLF G2 w88 online sports betting sorter is gentler than any conventional w88 online sports betting sorters, enabling healthier cells post-sort, especially for engineered lines, primary cells, and stem cells. All laser configurations afford <250 MESF sensitivity, along with forward and backscatter, providing as low as 1 μm resolution.

The ease of use and operation of the Wolf G2 utilizes disposable, aerosol-free microfluidic cartridges that allow for sterile sorting that protects the sample from the environment and scientists from the sample. The cartridges employ a piezoelectric actuator and operate at less than 2 psi allowing for gentle sorting that preserves viability and RNA integrity. Cartridges have a 100% disposable fluidic pathway, making it ideal for labs with many w88 online sports betting where cross-contamination or biohazard cleanup is an issue. The microfluidic cartridges are sterile and produce no aerosols, making it some of the safest sorting on the market. The cartridges also allow for maximum flexibility by allowing users to sort using their own buffers and media and only consume about 50 mL of sheath fluid in 1 day.

Note: users must purchase their own consumables, i.e. microfluidic sorting cartridges, calibration beads

Key features include:

• Two laser configurations, 488 w88 online sports betting and 561 w88 online sports betting
• A computer configured with WOLF viewer w88 online sports betting
• N1 Single-w88 online sports betting Dispenser module, 96-well plate
• CS1 w88 online sports betting/Sheath Chiller and Stirrer module