Core descriptions and w88 casino lists: COBRE – Smooth Muscle Plasticity

w88 casino Cytometry Center

The Fluorescence-Activated Cell Sorting/Flow Cytometry Shared Resource Laboratory (FACS/FCMSRL) is a state-of-the-art facility providing analytical fluorescence-based flow cytometry and cell sorting. It serves investigators from laboratories in the w88 casino School of Medicine and the w88 casino, Reno, in addition to other public and private laboratories in the area.

Director:Doug Redelman| (775) 784-6459

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• BD Biosciences FACSCalibur
• BD Biosciences Special Order w88 casino Product (SORP) LSRII digital cell analyzer with a High Throughput Sampler (HTS) unit for plates
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Single Cell Molecular Expression w88 casino (SCME) (COBRE w88 casino C)

The Single Cell Molecular Expression Core (SCME) provides investigators and other researchers in w88 casino System of Higher Education with automated single cell capture, quantitative PCR assays and targeted template enrichment, small noncoding RNA deep sequencing (sncRNA-Seq) or targeted DNA deep sequencing, ribosome profiling, gene of interest manipulation using ZFN’s, TALEN’s and CRISPR/Cas9 technologies, assistance in the design, execution and analysis of next generation deep sequencing, and genome editing experiments.

Director:Wei Yan| (775) 784-7765

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• Fluidigm C1 single w88 casino autoprep system
• Covaris M220 focused-ultrasonicator
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