Christine Cremo, Ph.D.

Professor, Biochemistry and w88 Biology Pharmacology

Summary

I have worked in the field of muscle proteins since my doctoral work on the muscarinic acetylcholine receptor in the heart. After working on fluorescent nucleotide derivatives for studies on skeletal myosin structure and function, I moved on to try to understand the regulation of the smooth muscle myosin ATPase by phosphorylation of the regulatory light chain. The focus has been on structure-function relationships. This work is ongoing, as well as a new focus on structure-function relationships of smooth muscle w88 kinase. This work has high relevance to several human diseases such as asthma, hypertension, and gastrointestinal disorders.

Research

  • Regulation of smooth muscle contraction.
  • Solution w88 interactions.
  • Single molecule w88 interactions.
  • Single molecule interactions of w88 kinase with cytoskeletal proteins.

Education

  • Ph.D. - Oregon State University
  • B.A., Biology - Lewis and Clark College

Selected publications

2013

  • Jackson DR Jr, Webb M, Stewart TJ, Phillips T, Carter M, w88, Baker JE. 2013. Sucrose increases the activation energy barrier for actin-w88 strong binding. Archives of Biochemistry and Biophysics. pii: S0003-9861(13)00386-X. doi: 10.1016/j.abb.2013.12.012. [ePub ahead of print] w88: 24370736.
  • Hong F, Facemyer KC, Carter MS, Jackson del R, Haldeman BD, Ruana N, Sutherland C, Walsh MP, w88, Baker JE. 2013. Kinetics of w88 kinase activation of smooth muscle myosin in an in vitro model system. Biochemistry. 52(47):8489-500. doi: 10.1021/bi401001x. ePub 2013 Nov 11. w88: 24144337; PubMed Central PMCID: PMC3886827.
  • Webb M, Jackson del R Jr, Stewart TJ, Dugan SP, Carter MS, w88, Baker JE. 2013. The w88 duty ratio tunes the calcium sensitivity and cooperative activation of the thin filament. Biochemistry. 52(37):6437-44. doi: 10.1021/bi400262h. ePub 2013 Sep 3. w88: 23947752.
  • Stewart TJ, Jackson DR Jr, Smith RD, Shannon SF, w88, Baker JE. 2013. Actin Sliding Velocities are Influenced by the Driving Forces of Actin-w88 Binding. Cellular and w88 Bioengineering. 6(1):26-37. PubMed PMID: 23606917; PubMed Central PMCID: PMC3627502.

2012

  • Ni S, Hong F, Haldeman BD, Baker JE, Facemyer KC, w88. 2012. Modification of interface between regulatory and essential light chains hampers phosphorylation-dependent activation of smooth muscle w88. Journal of Biological Chemistry. 287(26):22068-79. doi: 10.1074/jbc.M112.343491. ePub 2012 May 1. w88: 22549781; PubMed Central PMCID: PMC3381165.

2011

  • Sich NM, O'Donnell TJ, Coulter SA, John OA, Carter MS, w88, Baker JE. 2011. Effects of actin-w88 kinetics on the calcium sensitivity of regulated thin filaments. Journal of Biological Chemistry. 285(50):39150-9. doi: 10.1074/jbc.M110.142232. ePub 2010 Oct 2. w88: 20889979; PubMed CentralPMCID: PMC2998111.
  • Hong F, Haldeman BD, Jackson D, Carter M, Baker JE, w88. 2011. Biochemistry of smooth muscle w88 kinase. Archives of Biochemistry and Biophysics. 510(2):135-46. doi: 10.1016/j.abb.2011.04.018. ePub 2011 May 3. Review. w88: 21565153; PubMed Central PMCID: PMC3382066.
  • Jung HS, Billington N, Thirumurugan K, Salzameda B, w88, Chalovich JM, Chantler PD, Knight PJ. 2011. Role of the tail in the regulated state of w88 2. Journal of w88 Biology. 408(5):863-78. doi: 10.1016/j.jmb.2011.03.019. ePub 2011 Mar 23. PubMed PMID: 21419133; PubMed Central PMCID: PMC3776433.
  • Vileno B, Chamoun J, Liang H, Brewer P, Haldeman BD, Facemyer KC, Salzameda B, Song L, Li HC, w88, Fajer PG. 2011. Broad disorder and the allosteric mechanism of w88 II regulation by phosphorylation. Proceedings of the National Academy of Sciences U.S.A. 108(20):8218-23. doi: 10.1073/pnas.1014137108. ePub 2011 May 2. w88: 21536903; PubMed Central PMCID: PMC3100986.
  • Milton DL, Schneck AN, Ziech DA, Ba M, Facemyer KC, Halayko AJ, Baker JE, Gerthoffer WT, w88. 2011. Direct evidence for functional smooth muscle w88 II in the 10S self-inhibited monomeric conformation in airway smooth muscle cells. Proceedings of the National Academy of Sciences. 108(4):1421-6. doi: 10.1073/pnas.1011784108. ePub 2011 Jan 4. w88: 21205888; PubMed Central PMCID: PMC3029703.