Summary

• Major: Microbiology & Immunology
• Faculty Mentor: Dr. Dorothy Hudig
• Research Topic: Reduction of Natural Killer cell w88 casino games login by membrane bound non-supporting IgA antibody
• New Scholar: 2023 Cohort
• Graduating with a Baccalaureate Degree: 2025

Abstract

Viral pandemics, such as SARS-CoV-2, have caused extensive human suffering and mortality whilst efforts to mitigate such diseases have been insufficient. To improve control of infectious diseases, all mechanisms contributing to immunity must be further explored. This project will study a poorly understood mechanism of viral immunity: antibodies that bind to Natural Killer w88 casino games login and direct them to kill virally infected w88 casino games login, also known as antibody-dependent cellular cytotoxicity (ADCC). Enhancing this antibody-dependent cell-mediated cytotoxicity mechanism could improve the efficacy of life-saving vaccines. Ineffective antibodies for ADCC, unable to bind to NK w88 casino games login, may inhibit ADCC-dependent clearance of virally infected w88 casino games login. This is especially relevant in environments such as the nasal-associated lymphoid tissues (NALT), where considerable amounts of IgA, an ineffective ADCC antibody, are present. The experimental procedure consists of Daudi w88 casino games login, an immortalized line of B lymphocytes expressing the CD20 surface antigen, which are radiolabeled with 51Cr. These w88 casino games login are incubated in 96 well plates with the anti-CD20 IgG1 antibody GA101, which NK w88 casino games login are capable of recognizing with their CD16a receptors. Then, some of the targets will receive either IgG1 LALA, a variant of GA101 modified so that NK w88 casino games login can no longer recognize it, or IgA, which is an innately non-ADCC forming antibody. Our current results have shown that the sequential addition of ineffective antibody reduces ADCC significantly in both laboratories engineered NK-92 w88 casino games login as well as blood NK w88 casino games login. With this understanding, our next direction is to measure the ADCC inhibition following a simultaneous addition of antibodies.