Last summer, w88 online live casino IDeA Network of Biomedical Research Excellence, INBRE, in collaboration with w88 online live casino Bioinformatics Center hosted its third cohort for the Microbiome Undergraduate Summer Boot Camp. This three-week program introduced 19 students to a unique perspective in biomedical science. Participants developed skills in data analysis, laboratory safety and critical thinking. The curriculum combined hands-on wet-lab techniques, such as DNA sequencing with computational methods, offering a comprehensive introduction to biomedical research.
On day one, the boot camp launched with a networking lunch, an introduction to the gut microbiome and laboratory safety training all before visiting students settled into the dorms in Reno. To prepare for lab work, students completed hands-on instruction in the STAR lab. They learned how to handle chemical spills and use fume hoods – essential safety skills for the advanced laboratory procedures taught by Assistant Professor of Nutrition Steven Frese in the College of Agriculture, Biotechnology & Natural Resources and his graduate student, Matthew Bolino.
In the spirit of the 2024 Summer Olympics,the Frese Lab, which is also supported by the University’s Experiment Station unit, hosted its own “Laboratory Olympics,” challenging participants across four scientific events. The first event tested lab-related math skills. Next came a fast-paced dilution challenge, measuring pipetting accuracy and speed. The third event focused on precision pipetting, requiring steady hands to navigate smaller gel wells. Finally, the cumulative round brought it all together as participants applied their knowledge and crafted a master-mix solution. Armed with their newfound skills, students extracted microbial DNA from samples provided by volunteers inthe WOLFPACK Study. Students completed the DNA extractions and samples were sent to theIdaho State University Molecular Research Core Facility(RRID:SCR_012598) for 16S rRNA sequencing.
After a week and a half in Reno, the students moved to the University’s Lake Tahoe campus for data analysis. They learned coding in R, using packages like “DADA2,” “pyloseq” and “ggplot2” to create visually compelling graphs of microbial species diversity.
Students developed and refined individual research questions, exploring topics such as the relationship between household pets and the effects of food insecurity on microbiome health. Students were empowered to direct the research, tackling questions that held personal significance.
Beyond lab skills, the boot camp featured workshops and presentations that offered insight into the life of a student researcher, including how to apply to medical school, graduate student life and the importance of professionalism. The program was designed to foster connection through group projects while giving students time to explore Reno and Tahoe. Group hikes, park visits, movie nights and shared meals created lasting memories and forged strong friendships, laying the foundation for future professional relationships.
Nearing the end of the boot camp, students prepared for the 2024 Wolf Pack Discoveries Symposium. For many, this was their first time presenting a scientific poster. To prepare, students engaged in workshops, mock presentations and multiple stages of feedback, honing their science communication skills. The result was that all students confidently presented their methods and the significance of their research to other students and the public – a fitting finale to the program.
The 2024 Microbiome Undergraduate Summer Boot Camp was a resounding success. Over three immersive weeks, aspiring researchers gained hands-on experience in the biomedical research, from bench work to data analysis to presenting their findings. Along the way, they built lasting connections with their peers and faculty who dedicated their time and expertise to the program.
The students wish to give special thanks to Steven Frese, Ph.D., and Matthew Bolino for their invaluable training in DNA sequencing techniques and engaging talks on microbiome science. They also extend their heartfelt gratitude to Juli Petereit, Ph.D., whose vision and unwavering support made this program possible. Her kindness, dedication to student success and inspiring mentorship continue to bring out the best in everyone she guides.
w88 online live casino INBRE is happy to announce that it will be offering its fourth cohort this summer, July 21-Aug. 8, 2025, which will include many of the same exciting opportunities along with new program updates. Applications are now open and due April 24. Seats are limited and early applications are encouraged. If accepted, the program is at no cost for students enrolled in NSHE institutions and includes room and board.
About the authors
Jose Castillo and Simone Jacot are undergraduate researchers who participated in the Microbiome Research summer program. Castillo is a junior majoring in biology at w88 online live casino State University, and Jacot is a sophomore majoring in nutritional science at the w88 online live casino, Reno.
